Goo Youn-Kyoung, Jia Honglin, Terkawi Mohamad Alaa, Aboge Gabriel Oluga, Yamagishi Junya, Nishikawa Yoshifumi, Kim Suk, Jang Hyung-Kwan, Fujisaki Kozo, Xuan Xuenan
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
Exp Parasitol. 2009 Nov;123(3):273-6. doi: 10.1016/j.exppara.2009.07.017. Epub 2009 Aug 6.
Babesia gibsoni causes canine babesiosis. Here, we describe the identification and characterization of a novel gene, BgP22, containing an open reading frame of 621bp and encoding a 22-kDa protein from B. gibsoni, as a serodiagnostic candidate. The recombinant BgP22 (rBgP22) was expressed and used as an antigen to produce anti-rBgP22 sera in mice. Using these anti-rBgP22 sera, a native 22-kDa protein was recognized by Western blot analysis and observed in the membrane of the parasites by immunofluorescent antibody tests (IFAT). The enzyme-linked immunosorbent assay (ELISA) using the rBgP22 detected specific antibodies to this protein in the sera of dogs experimentally and naturally infected with B. gibsoni in chronic stage. Furthermore, it did not show a cross reaction with the closely related apicomplexan parasites, indicating that the rBgP22 could be used as a diagnostic antigen for a detection of the chronic carrier stages of B. gibsoni infection.
吉氏巴贝斯虫可引发犬巴贝斯虫病。在此，我们描述了一个新基因BgP22的鉴定与特性，该基因含有一个621bp的开放阅读框，编码来自吉氏巴贝斯虫的一种22kDa蛋白，作为一种血清学诊断候选物。重组BgP22（rBgP22）被表达出来，并用作抗原在小鼠体内产生抗rBgP22血清。使用这些抗rBgP22血清，通过蛋白质免疫印迹分析识别出一种天然的22kDa蛋白，并通过免疫荧光抗体试验（IFAT）在寄生虫膜中观察到该蛋白。使用rBgP22的酶联免疫吸附测定（ELISA）在实验性和自然感染吉氏巴贝斯虫慢性期的犬血清中检测到针对该蛋白的特异性抗体。此外，它与密切相关的顶复门寄生虫没有交叉反应，表明rBgP22可作为检测吉氏巴贝斯虫感染慢性携带阶段的诊断抗原。
