Numazaki K, Goldman H, Seemayer T A, Wong I, Wainberg M A
McGill University-Montreal Children's Hospital Research Institute, Québec, Canada.
In Vivo. 1990 Jan-Feb;4(1):49-54.
Several viruses are implicated in the pathogenesis of pancreatic beta cell destruction and the onset of insulin-dependent (type 1) diabetes mellitus (IDDM). However, it is still controversial as to whether viral infection can exert direct cytopathic effects on beta cells. We have studied the effect of infection by human cytomegalovirus (CMV) and rubella virus on human fetal pancreatic islets in tissue culture. Although morphological changes were observed in CMV-inoculated islet cells, such infection did not lead to changes in levels of secreted immunoreactive insulin. On the other hand, infection by rubella virus did lead to significantly lower levels of immunoreactive insulin in the absence of demonstrable cytopathic effect. These results indicate that viral infection of human pancreatic islet tissue can occur as demonstrated through tissue culture techniques, and that such infection leads to changes in levels of secreted insulin in some cases but not others.
几种病毒与胰腺β细胞破坏及胰岛素依赖型（1型）糖尿病（IDDM）的发病机制有关。然而，病毒感染是否能对β细胞产生直接细胞病变效应仍存在争议。我们研究了人巨细胞病毒（CMV）和风疹病毒感染对组织培养中的人胎儿胰岛的影响。虽然在接种CMV的胰岛细胞中观察到形态学变化，但这种感染并未导致分泌的免疫反应性胰岛素水平发生变化。另一方面，风疹病毒感染在没有明显细胞病变效应的情况下确实导致免疫反应性胰岛素水平显著降低。这些结果表明，通过组织培养技术已证实人胰岛组织可发生病毒感染，且这种感染在某些情况下会导致分泌胰岛素水平的变化，而在其他情况下则不会。
