Numazaki K, Goldman H, Wong I, Wainberg M A
McGill University-Montreal Children's Hospital Research Institute, Quebec, Canada.
Am J Clin Pathol. 1989 Apr;91(4):446-51. doi: 10.1093/ajcp/91.4.446.
A high incidence of insulin-dependent diabetes mellitus (IDDM) has been reported in children and young adults previously afflicted with congenital rubella syndrome (CRS). The authors have studied the effect of rubella virus infection on human pancreatic islet cells in tissue culture. These experiments were performed with the use of both monolayers and free-floating human fetal islets of Langerhans tissue. Levels of production of immunoreactive insulin by islet cells that had been infected by rubella virus were lower than those observed in control cultures, under conditions of high glucose concentration (11.1 mmol/L) in the medium. The presence of rubella viral antigens in human pancreatic beta and non-beta cells was demonstrated by double-label immunofluorescence. These results suggest that rubella virus can infect human pancreatic islet cells and that such infection may lead to significant reductions in levels of secreted insulin.
据报道，先前患有先天性风疹综合征（CRS）的儿童和年轻人中胰岛素依赖型糖尿病（IDDM）的发病率很高。作者研究了风疹病毒感染对组织培养中的人胰岛细胞的影响。这些实验使用了单层培养和游离漂浮的人胎儿朗格汉斯胰岛组织。在培养基中高葡萄糖浓度（11.1 mmol/L）的条件下，感染风疹病毒的胰岛细胞产生免疫反应性胰岛素的水平低于对照培养物中观察到的水平。通过双标记免疫荧光证明了人胰腺β细胞和非β细胞中存在风疹病毒抗原。这些结果表明，风疹病毒可感染人胰岛细胞，且这种感染可能导致分泌胰岛素水平显著降低。
