Bordoni A, Tantini B, Clo C, Turchetto E
Dipartimento di Biochimica, Universita' di Bologna, Italy.
Cardioscience. 1990 Dec;1(4):235-9.
Polyunsaturated fatty acids are involved at several steps in the turnover of phosphatidylinositol mediated by alpha 1-adrenoceptors. We have cultured neonatal rat cardiomyocytes in a medium containing docosahexaenoic acid (C22:6n3) and have investigated the effects produced by the change in the fatty acid composition of phosphatidylinositol on alpha 1-adrenoceptor-mediated phosphatidylinositol metabolism. The experimental cells, in which phosphatidylinositol was significantly enriched in docosahexaenoic acid and depleted in arachidonic acid, showed a reduced ability to incorporate labeled inositol into phospholipids in comparison with control cells, both in basal conditions and after stimulation by phenylephrine. This decreased incorporation led to a reduced availability of inositol phospholipids, substrates of phospholipase C, and to a reduced production of inositol phosphates under basal conditions.
多不饱和脂肪酸参与了由α1 - 肾上腺素能受体介导的磷脂酰肌醇代谢的多个步骤。我们在含有二十二碳六烯酸（C22:6n3）的培养基中培养新生大鼠心肌细胞，并研究了磷脂酰肌醇脂肪酸组成的变化对α1 - 肾上腺素能受体介导的磷脂酰肌醇代谢产生的影响。与对照细胞相比，实验细胞中磷脂酰肌醇显著富含二十二碳六烯酸且花生四烯酸减少，在基础条件下以及去氧肾上腺素刺激后，其将标记的肌醇掺入磷脂的能力均降低。这种掺入减少导致磷脂酶C的底物肌醇磷脂的可用性降低，并导致基础条件下肌醇磷酸的产生减少。
