Schroeter Annett, Kiselev Mikhail A, Hauss Thomas, Dante Silva, Neubert Reinhard H H
Martin-Luther-Universität Halle-Wittenberg, Institute of Pharmacy, Department of Pharmaceutics and Biopharmaceutics, Halle, Germany.
Biochim Biophys Acta. 2009 Oct;1788(10):2194-203. doi: 10.1016/j.bbamem.2009.07.024. Epub 2009 Aug 6.
This research paper provides direct evidence concerning the localisation of free fatty acids in stratum corneum lipid model membranes. We employed partially deuterated free fatty acids to gain further information about the assembly of a stratum corneum lipid model membrane based on a ceramide of the phytosphingosine-type (ceramide [AP]) with particular respect to the position of the deuterated groups of the free fatty acids. The application of behenic-22,22,22-d(3)-acid and cerotic-12,12,13,13-d(4)-acid confirmed that the short-chain ceramide [AP] forces the longer-chained free fatty acids to incorporate into the bilayer created by ceramide [AP]. The ceramide [AP] molecules determine the structural assembly of this model membrane and obligate the long-chain free fatty acids to either arrange inside this formation or to separate as a fatty acid rich phase.
这篇研究论文提供了关于游离脂肪酸在角质层脂质模型膜中定位的直接证据。我们使用了部分氘代的游离脂肪酸，以获取更多关于基于植物鞘氨醇型神经酰胺（神经酰胺[AP]）构建的角质层脂质模型膜组装的信息，特别是关于游离脂肪酸氘代基团的位置。山嵛酸-22,22,22-d(3)-酸和蜡酸-12,12,13,13-d(4)-酸的应用证实，短链神经酰胺[AP]促使长链游离脂肪酸掺入由神经酰胺[AP]形成的双层中。神经酰胺[AP]分子决定了该模型膜的结构组装，并迫使长链游离脂肪酸要么排列在该结构内部，要么分离形成富含脂肪酸的相。
