Jin Xi, Chen Qian, Tang Shu-Sheng, Zou Jia-Jie, Chen Kai-Pao, Zhang Ting, Xiao Xi-Long
Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, PR China.
Toxicol In Vitro. 2009 Oct;23(7):1209-14. doi: 10.1016/j.tiv.2009.07.038. Epub 2009 Aug 7.
Quinocetone, a new quinoxaline 1,4-dioxide derivative, has been approved as an animal growth promoter in China since 2003. To investigate the genotoxicity of quinocetone in vitro, its effects on the extent of DNA injury in human hepatoma (HepG2) cells accompanied by chromosomal damage and genomic DNA alterations were tested. The cell viability test indicated that quinocetone inhibited cell proliferation as a function of dose and time. In the comet assay, significant DNA fragment migration was observed in a dose-dependent manner. A dose-dependent increase of the micronucleated (MN) cell frequency was shown in cytokinesis-block micronucleus (CBMN) test. The gain/loss of randomly amplified polymorphic DNA (RAPD) bands and the change of band intensity in RAPD profiles were obtained after HepG2 cells were exposed to quinocetone at concentrations of 1.25, 2.5 and 5 microg/mL. The results demonstrated that quinocetone exerted genotoxic effects on HepG2 cells. Thus, the use of quinocetone as a growth promoter in animal feed should be seriously considered.
喹烯酮是一种新型喹喔啉 1,4-二氧化物衍生物，自 2003 年起在中国被批准用作动物生长促进剂。为了研究喹烯酮的体外遗传毒性，测试了其对人肝癌（HepG2）细胞中 DNA 损伤程度的影响，以及伴随的染色体损伤和基因组 DNA 改变。细胞活力测试表明，喹烯酮抑制细胞增殖，呈剂量和时间依赖性。在彗星试验中，观察到显著的 DNA 片段迁移，呈剂量依赖性。在胞质分裂阻断微核（CBMN）试验中，微核（MN）细胞频率呈剂量依赖性增加。将 HepG2 细胞暴露于浓度为 1.25、2.5 和 5 μg/mL 的喹烯酮后，获得了随机扩增多态性 DNA（RAPD）条带的增减以及 RAPD 图谱中条带强度的变化。结果表明，喹烯酮对 HepG2 细胞具有遗传毒性作用。因此，应认真考虑在动物饲料中使用喹烯酮作为生长促进剂的问题。
