Furuhashi Takeshi, Schwarzinger Clemes, Miksik Ivan, Smrz Miloslav, Beran Anton
Department of Evolutionary Biology, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria.
Comp Biochem Physiol B Biochem Mol Biol. 2009 Nov;154(3):351-71. doi: 10.1016/j.cbpb.2009.07.011. Epub 2009 Aug 7.
Biomineralization research on mollusc shells has mostly focused on nacre formation. Chitin, silk fibroin protein, and acidic macromolecules are important components for shell formation. Although the principle concept behind shell calcification was developed many years ago, the individual components have not been well scrutinized. Besides that, Mollusca are the second largest invertebrate phylum, but comprehensive biochemical research involving a comparison of different taxa is still rare. This study reconsiders the above three components with adding some biochemical data of aculiferans. The presence of chitin in polyplacophorans sclerites was confirmed by IR and pyrolysis GC/MS. DMMB staining data inferred that sulphated groups present in aplacophoran cuticle but not in polyplacophorans cuticle. These insight suggested importance of comparison between acuriferans and conchiferans.
对软体动物贝壳的生物矿化研究大多集中在珍珠层的形成上。几丁质、丝素蛋白和酸性大分子是贝壳形成的重要成分。尽管贝壳钙化背后的基本概念早在多年前就已提出，但各个成分尚未得到充分研究。除此之外，软体动物是第二大无脊椎动物门，但涉及不同分类群比较的全面生化研究仍然很少。本研究通过补充一些有壳类动物的生化数据，重新审视了上述三种成分。通过红外光谱和热解气相色谱/质谱法证实了多板纲动物骨片中有几丁质存在。二甲基甲酰胺二甲苯蓝染色数据推断，无板纲动物角质层中存在硫酸化基团，而多板纲动物角质层中不存在。这些见解表明了有壳类动物和贝类动物之间比较的重要性。
