Donaghy K, Scholfield C N
School of Biomedical Science, Queens University, Belfast, UK.
Neuroreport. 1990 Oct;1(2):123-5. doi: 10.1097/00001756-199010000-00010.
Transverse slices of rat hippocampus were used to test the effect of adenosine on recovery of synaptically-evoked potentials after anoxia. To produce conditions better resembling ischaemia, glucose was omitted during the period of anoxia. Anoxia abolished all electrical activity within 5 min. The responses recovered completely after short periods of anoxia (3 min), but the amount of recovery was progressively reduced with longer exposures. The recovery was enhanced by the presence of 100 microM adenosine: the exposure time giving a 50% recovery of the response was 17.6 +/- 1.8 min in normal solution and 29.0 +/- 4.1 min with adenosine. These results are consistent with the idea that adenosine offers some protection from anoxia.
采用大鼠海马横切片来测试腺苷对缺氧后突触诱发电位恢复的影响。为营造更接近缺血的条件，缺氧期间省去葡萄糖。缺氧在5分钟内使所有电活动消失。短时间缺氧（3分钟）后反应完全恢复，但随着缺氧时间延长，恢复量逐渐减少。100微摩尔的腺苷可增强恢复：在正常溶液中使反应恢复50%的暴露时间为17.6±1.8分钟，而加入腺苷后为29.0±4.1分钟。这些结果与腺苷能提供一定缺氧保护的观点一致。
