Aducci P, Marra M, Ballio A
Department of Biology, University of Rome Tor Vergata, Italy.
Symp Soc Exp Biol. 1990;44:111-7.
The first event in the mode of action of the fungal phytotoxin fusicoccin is the binding to specific receptors located at the plasma membrane. They are very likely related to the presence of endogenous compounds able to compete with FC for binding to its receptors. Some biochemical properties of these proteins conform to criteria commonly used for receptors. A functional relationship between fusicoccin receptors and the plasma membrane H(+)-ATPase which is assumed to be the final physiological target of fusicoccin, has been recently suggested. New support in favour of this relationship has been sought by studying a model system prepared by insertion into liposomes of preparations from maize tissues of the two proteins. The model system is capable of H(+)-translocation on ATP addition and the translocation is markedly stimulated by fusicoccin. Thus, it represents a promising tool for the understanding of fusicoccin signal transduction at the molecular level, since it can assist in the detection and assay of the transduction chain components contained in the impure protein preparations.
真菌植物毒素壳梭孢菌素作用模式的首个事件是与位于质膜上的特定受体结合。它们很可能与能够与壳梭孢菌素竞争结合其受体的内源性化合物的存在有关。这些蛋白质的一些生化特性符合通常用于受体的标准。最近有人提出壳梭孢菌素受体与质膜H(+) -ATP酶之间存在功能关系，而质膜H(+) -ATP酶被认为是壳梭孢菌素的最终生理靶点。通过研究一个模型系统来寻求对这种关系的新支持，该模型系统是通过将玉米组织中这两种蛋白质的制剂插入脂质体而制备的。该模型系统在添加ATP时能够进行H(+)转运，并且壳梭孢菌素能显著刺激这种转运。因此，它是在分子水平上理解壳梭孢菌素信号转导的一个有前途的工具，因为它有助于检测和测定不纯蛋白质制剂中所含的转导链成分。
