Hooley R, Beale M H, Smith S J
Department of Agricultural Sciences, University of Bristol, UK.
Symp Soc Exp Biol. 1990;44:79-86.
Gibberellins (GAs) are a class of plant hormones involved in the regulation of plant growth and development. A useful model system for studying GA-action is the aleurone layer of cereal grain. In aleurone, GA induces a considerable increase in the rates of transcription of alpha-amylase genes, and this effect can be inhibited by another plant hormone abscisic acid. We anticipate that perception of GA by aleurone cells involves an interaction between the ligand and specific receptors and that this event leads to the observed stimulation in transcription of alpha-amylase, and probably other, aleurone genes. Hormone binding assays with aleurone have not revealed a candidate receptor, and the only aspect of signal transduction that is becoming understood is the likely interaction between specific trans-acting factors and cis-elements in the GA-regulation of alpha-amylase gene transcription. This paper describes the development of novel ways for identifying GA-receptors in aleurone protoplasts of Avena fatua that are based on the use of affinity and antibody probes in functional in vivo assays for GA-receptors. The implications of our current findings are discussed in relation to models of GA-action in aleurone.
赤霉素（GAs）是一类参与植物生长发育调控的植物激素。研究赤霉素作用的一个有用的模型系统是谷物的糊粉层。在糊粉层中，赤霉素可使α-淀粉酶基因的转录速率显著增加，而这一效应可被另一种植物激素脱落酸抑制。我们预计，糊粉层细胞对赤霉素的感知涉及配体与特定受体之间的相互作用，并且这一事件会导致观察到的α-淀粉酶以及可能其他糊粉层基因转录的刺激。用糊粉层进行的激素结合试验尚未揭示候选受体，而目前正在被理解的信号转导的唯一方面是特定反式作用因子与α-淀粉酶基因转录的赤霉素调控中的顺式元件之间可能的相互作用。本文描述了基于在GA受体的功能性体内试验中使用亲和探针和抗体探针来鉴定燕麦糊粉层原生质体中GA受体的新方法的开发。我们当前研究结果的意义将结合糊粉层中GA作用的模型进行讨论。
