β-肾上腺素能激动剂刺激下环磷酸腺苷升高与粘蛋白分泌之间的解离。

Dissociation between cyclic AMP rise and mucin secretion in response to a beta-adrenergic agonist.

作者信息

Bradbury N A, Dormer R L, McPherson M A

机构信息

Department of Medical Biochemistry, University of Wales College of Medicine, Cardiff, U.K.

出版信息

Acta Univ Carol Med (Praha). 1990;36(1-4):55-7.

PMID:1966660

Abstract

A novel hypotonic swelling method has been used to introduce cyclic AMP phosphodiesterase (cAMP PDE) into intact rat submandibular acini. Acini swollen in the presence of cAMP PDE showed no cyclic AMP rise in response to the beta-agonist isoproterenol; however, stimulation of mucin secretion was not impaired. Control experiments showed that acini swollen in the absence of cAMP PDE showed normal cyclic AMP rise and mucin secretion responses. The results showing a dissociation between cyclic AMP rise and mucin secretion suggest that cyclic AMP is not the sole mediator of beta-adrenergic stimulation of epithelial cell secretion. The method of intracellular manipulation described will allow (i) investigation of key regulators of secretory events, shown to be defective in CF cells. (ii) determination of the biochemical function of proteins which are candidates for the biochemical defect in CF.

摘要

一种新型的低渗肿胀方法已被用于将环磷酸腺苷磷酸二酯酶（cAMP PDE）导入完整的大鼠下颌下腺腺泡。在cAMP PDE存在的情况下肿胀的腺泡对β-激动剂异丙肾上腺素无环磷酸腺苷升高反应；然而，粘蛋白分泌的刺激并未受损。对照实验表明，在不存在cAMP PDE的情况下肿胀的腺泡显示出正常的环磷酸腺苷升高和粘蛋白分泌反应。显示环磷酸腺苷升高与粘蛋白分泌之间解离的结果表明，环磷酸腺苷不是β-肾上腺素能刺激上皮细胞分泌的唯一介质。所描述的细胞内操作方法将允许：（i）研究分泌事件的关键调节因子，已证明这些调节因子在囊性纤维化（CF）细胞中有缺陷。（ii）确定作为CF生化缺陷候选蛋白的生化功能。