Marmara University, Faculty of Medicine, Department of Microbiology, Istanbul, Turkey.
Research Service, VA Medical Center West Los Angeles, Los Angeles, CA 90073, USA.
Int J Syst Evol Microbiol. 2010 May;60(Pt 5):1013-1016. doi: 10.1099/ijs.0.015909-0. Epub 2009 Aug 7.
Two strains of previously unknown Gram-stain-positive, anaerobic, coccus-shaped bacteria from human wound specimens were characterized using phenotypic and molecular taxonomic methods. Comparative 16S rRNA gene sequencing studies and distinguishable biochemical characteristics demonstrated that these two unknown strains, WAL 1855C(T) and WAL 2038E, are genotypically homogeneous and constitute a novel lineage within Clostridium cluster XIII. There was 13-14 % 16S rRNA gene sequence divergence between the novel strains and the most closely related species, Parvimonas micra, Finegoldia magna and species of Helcococcus. Based on the phenotypic and phylogenetic findings, a novel genus and species, Murdochiella asaccharolytica gen. nov., sp. nov., are proposed. Strain WAL 1855C(T) (=ATCC BAA-1631(T) =CCUG 55976(T)) is the type strain of Murdochiella asaccharolytica.
从人类伤口标本中分离到两株先前未知的革兰氏阳性、厌氧、球菌状细菌，采用表型和分子分类学方法对其进行了表征。比较 16S rRNA 基因测序研究和可区分的生化特征表明，这两种未知菌株 WAL 1855C(T) 和 WAL 2038E 在基因型上是同质的，构成了梭菌属 13 簇中的一个新谱系。新型菌株与最密切相关的种，即 Parvimonas micra、Finegoldia magna 和 Helcococcus 种之间的 16S rRNA 基因序列差异为 13-14%。基于表型和系统发育发现，提出了一个新属和新种，即 Murdochiella asaccharolytica gen. nov.，sp. nov.。菌株 WAL 1855C(T)=ATCC BAA-1631(T)=CCUG 55976(T)是 Murdochiella asaccharolytica 的模式株。
