Kumar K K, Ambekar G R, Shewale J G
Hindustan Antibiotics Ltd., Pimpri, Pune.
Hindustan Antibiot Bull. 1990 Aug-Nov;32(3-4):84-7.
Penicillin G acylase was immobilized on numerous cation exchange resins and hydrophobic adsorbents. Amberlite XAD-7 was the matrix of choice among the matrices studied for the immobilization of enzyme. Binding of 96.8% and expression of 82.6% of the penicillin G acylase was achieved on XAD-7. Penicillin G acylase immobilised on XAD-7 was used for 80 cycles for the production of 6-PA in a stirred tank reactor.
青霉素G酰化酶被固定在多种阳离子交换树脂和疏水吸附剂上。在研究的用于固定化酶的基质中，Amberlite XAD - 7是首选基质。在XAD - 7上实现了96.8%的结合率和82.6%的青霉素G酰化酶表达率。固定在XAD - 7上的青霉素G酰化酶在搅拌釜式反应器中用于生产6 - 氨基青霉烷酸80个循环。
