通过免疫组织化学和对石蜡包埋样本进行聚合酶链反应诊断小型反刍动物胎盘病原体

Diagnosis of placental pathogens in small ruminants by immunohistochemistry and PCR on paraffin-embedded samples.

作者信息

Navarro J A, Ortega N, Buendia A J, Gallego M C, Martínez C M, Caro M R, Sánchez J, Salinas J

机构信息

Departamento de Anatomía y Anatomía Patológica Comparadas, Facultad de Veterinaria, Universidad de Murcia, Murcia, Spain.

出版信息

Vet Rec. 2009 Aug 8;165(6):175-8. doi: 10.1136/vr.165.6.175.

DOI:10.1136/vr.165.6.175

PMID:19666916

Abstract

A histological study was carried out on 58 formalin-fixed and paraffin-embedded samples of placenta from sheep and goats that had aborted, and the placental lesions were graded. Sequential histological sections of each cotyledon were then immunostained with specific antibodies and used for PCR detection of Chlamydophila abortus, Coxiella burnetii, Salmonella Abortusovis, Brucella melitensis, Listeria monocytogenes and Toxoplasma gondii. Most of the cotyledons showed different degrees of placentitis. The proportional agreement between the two techniques was 0.879 (kappa value 0.746). C abortus was the most prevalent pathogen. Mixed infections were common.

摘要

对58份来自流产绵羊和山羊的福尔马林固定石蜡包埋胎盘样本进行了组织学研究，并对胎盘病变进行了分级。然后，用特异性抗体对每个子叶的连续组织切片进行免疫染色，并用于检测流产嗜衣原体、伯氏考克斯体、绵羊流产沙门氏菌、羊布鲁氏菌、单核细胞增生李斯特菌和弓形虫的PCR检测。大多数子叶显示出不同程度的胎盘炎。两种技术之间的比例一致性为0.879（kappa值为0.746）。流产嗜衣原体是最常见的病原体。混合感染很常见。