Koo Ja Seung, Jung Woohee, Yang Woo Ick
Department of Pathology, Yonsei University Health System, Seoul, South Korea.
Int J Surg Pathol. 2011 Aug;19(4):425-32. doi: 10.1177/1066896909335821. Epub 2009 Aug 10.
Cases of breast cancer showing human epidermal growth factor receptor-2 (HER-2) protein overexpression without corresponding gene amplification have been found in immunohistochemistry (IHC) and fluorescent in situ hybridization (FISH) results. We investigated the clinicopathological characteristics of cases showing HER-2 protein overexpression breast cancer without gene amplification (IHC3+/FISH-) and compared them to cases showing HER-2 protein overexpression breast cancer with gene amplification (IHC3+/FISH+). This study was conducted on 90 patients with HER-2 IHC3+ breast cancer. The clinicopathological factors analyzed included tumor size, histological grade, nuclear grade, nodal involvement, and hormone receptor status. HER-2 IHC3+/FISH- breast cancer was found in 14 of 84 tumors (16.7%) and showed a statistically significant lower histological and nuclear grade (P = .000) and higher expression of estrogen receptors (ERs) and progesterone receptors (PRs) (P = .006) than IHC3+/FISH+ breast cancer. In conclusion, HER-2 IHC3+/FISH- breast cancer could be a subgroup showing lower histological/nuclear grade and higher expression of ERs/PRs.
在免疫组织化学（IHC）和荧光原位杂交（FISH）结果中发现了人类表皮生长因子受体2（HER-2）蛋白过度表达但无相应基因扩增的乳腺癌病例。我们研究了HER-2蛋白过度表达但无基因扩增（IHC3+/FISH-）的乳腺癌病例的临床病理特征，并将其与HER-2蛋白过度表达且有基因扩增（IHC3+/FISH+）的乳腺癌病例进行比较。本研究对90例HER-2 IHC3+乳腺癌患者进行。分析的临床病理因素包括肿瘤大小、组织学分级、核分级、淋巴结受累情况和激素受体状态。在84个肿瘤中有14个（16.7%）为HER-2 IHC3+/FISH-乳腺癌，与IHC3+/FISH+乳腺癌相比，其组织学和核分级在统计学上显著更低（P = .000），雌激素受体（ERs）和孕激素受体（PRs）的表达更高（P = .006）。总之，HER-2 IHC3+/FISH-乳腺癌可能是一个组织学/核分级较低且ERs/PRs表达较高的亚组。
