球形角膜的组织病理学和免疫组织化学研究。
Histopathologic and immunohistochemical studies of keratoglobus.
作者信息
Meghpara Beeran, Nakamura Hiroshi, Vemuganti Geeta K, Murthy Somasheila I, Sugar Joel, Yue Beatrice Y J T, Edward Deepak P
机构信息
Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, USA.
出版信息
Arch Ophthalmol. 2009 Aug;127(8):1029-35. doi: 10.1001/archophthalmol.2009.184.
OBJECTIVE
To examine histopathologic and immunohistochemical features of human corneal buttons from patients who developed keratoglobus.
METHODS
Nine corneal buttons were obtained during penetrating keratoplasty from patients with keratoglobus. Histologic features were examined using hematoxylin-eosin-stained sections. Immunohistochemical staining for alpha1-proteinase inhibitor, Sp1, and matrix metalloproteinases 1, 2, and 3 was performed, with 2 normal and 2 corneal sections with keratoconus as controls.
RESULTS
Hematoxylin-eosin staining revealed diffuse stromal thinning and focal disruptions in Bowman's layer in all keratoglobus specimens. Similar abnormal immunostaining results for alpha1-proteinase inhibitor and Sp1 were detected in keratoglobus and keratoconus at their respective active disease sites. Immunostaining for matrix metalloproteinases 1, 2, and 3 was significantly more intense in corneas with keratoglobus than in normal controls. Matrix metalloproteinase staining intensity was especially prominent in areas where the underlying Bowman's layer was disrupted.
CONCLUSIONS
Histological features in our keratoglobus specimens are consistent with previous reports. The similarities in immunohistochemical labeling between keratoglobus and keratoconus suggest that these entities may share common mechanisms that are involved in stromal thinning.
目的
研究发生圆锥角膜患者的人角膜植片的组织病理学和免疫组化特征。
方法
从圆锥角膜患者穿透性角膜移植术中获取9个角膜植片。使用苏木精-伊红染色切片检查组织学特征。对α1-蛋白酶抑制剂、Sp1以及基质金属蛋白酶1、2和3进行免疫组化染色，以2个正常角膜切片和2个圆锥角膜切片作为对照。
结果
苏木精-伊红染色显示，所有圆锥角膜标本均有弥漫性基质变薄和Bowman层的局灶性破坏。在圆锥角膜和圆锥角膜各自的活动病变部位，检测到α1-蛋白酶抑制剂和Sp1的免疫染色结果异常相似。圆锥角膜角膜中基质金属蛋白酶1、2和3的免疫染色明显强于正常对照。基质金属蛋白酶染色强度在下方Bowman层被破坏的区域尤为突出。
结论
我们的圆锥角膜标本的组织学特征与先前报道一致。圆锥角膜和圆锥角膜在免疫组化标记上的相似性表明，这些实体可能共享参与基质变薄的共同机制。
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