Institute of Molecular Biology, Academia Sinica, Nankang, Taipei, Taiwan.
RNA Biol. 2009 Sep-Oct;6(4):402-5. doi: 10.4161/rna.6.4.9320. Epub 2009 Sep 21.
Endoribonuclease E, 3'-5' exoribonuclease polynucleotide phosphorylase, RhlB RNA helicase and enolase form a multienzyme complex (the "degradosome") playing an important role in RNA processing and decay in Escherichia coli. Although a number of proteins that occasionally co-purify with the E. coli degradosome in non-stoichiometric amounts were initially viewed as insignificant constituents of this complex, recent studies suggest that, in fact, some of these copurifying proteins are involved in modulation of degradosome composition, activity or specificity during bacterial adaptation to changing environments. Here we briefly review these findings and discuss their implications for understanding the multifaceted mechanisms controlling degradosome functions in vivo.
内切核酸酶 E、3′-5′外切核酸酶多核苷酸磷酸化酶、RhlB RNA 解旋酶和烯醇化酶形成一个多酶复合物（“降解体”），在大肠杆菌中发挥重要的 RNA 加工和降解作用。尽管最初认为与大肠杆菌降解体在非化学计量比例下偶尔共纯化的许多蛋白质是该复合物的无足轻重的组成部分，但最近的研究表明，事实上，这些共纯化的蛋白质中的一些参与了降解体组成、活性或特异性的调节，以适应不断变化的环境。在这里，我们简要回顾这些发现，并讨论它们对理解体内控制降解体功能的多方面机制的意义。
