延伸对呼肠孤病毒双顺反子mRNA翻译的影响。
Effects of elongation on the translation of a reovirus bicistronic mRNA.
作者信息
Fajardo J E, Shatkin A J
机构信息
Center for Advanced Biotechnology and Medicine, Piscataway, N.J.
出版信息
Enzyme. 1990;44(1-4):235-43. doi: 10.1159/000468761.
The S1 species of reovirus mRNA contains two overlapping open reading frames. Both are utilized in either virus-infected or S1 DNA transfected mammalian cells, resulting in two different polypeptides from a single mRNA. Consistent with ribosome scanning, expression of the downstream reading frame was increased by sequence changes that diminished the consensus around the upstream initiator site. However, the upstream product was not decreased by the same changes, suggesting that its synthesis is rate-limited at elongation. The results suggest a model for regulating bicistronic mRNA translation in which the ribosomes in one reading frame interfere with the movement of ribosomes in the other frame due to different rates of elongation.
呼肠孤病毒mRNA的S1物种包含两个重叠的开放阅读框。在病毒感染或S1 DNA转染的哺乳动物细胞中，这两个阅读框都会被利用，从而从单个mRNA产生两种不同的多肽。与核糖体扫描一致，下游阅读框的表达通过减少上游起始位点周围共有序列的序列变化而增加。然而，相同的变化并没有降低上游产物，这表明其合成在延伸阶段受到速率限制。这些结果提示了一种双顺反子mRNA翻译调控模型，其中一个阅读框中的核糖体由于延伸速率不同而干扰另一个阅读框中核糖体的移动。
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