核酶：酶促反应单底物、双中间体可逆反应机制的解析解
Ribozymes: analytical solution of the one-substrate, two-intermediate reversible scheme for enzyme reactions.
作者信息
Toti Paolo, Sbordone Ludovico, Sbordone Carolina, Bauer Carlo
机构信息
Unità di Biochimica, Dipartimento di Biologia, Università di Pisa, 56127 Pisa, Italy.
出版信息
J Biol Phys. 2006 Dec;32(6):473-88. doi: 10.1007/s10867-006-9030-z. Epub 2007 Jan 27.
Abstract
The paper presents a kinetic analysis of a reversible enzymatic reaction Sright arrow over left arrowP involving two intermediate compounds under the condition E >> S + P. For the case of mono-exponential behavior, we derive an equation for k (obs) as a function of E, which emphasizes the pitfalls of oversimplifying kinetic schemes (such as the Michaelis-Menten model) for ribozyme studies. This novel apparent rate constant, which has been arrived at through mechanistic considerations, is analyzed, and the characteristic parameters obtained. The equation, which seems to fit experimental data better than conventional approximations, is used to analyze a single turnover study on an ADC1 ribozyme drawn from hepatitis delta virus RNA. The microscopic kinetic constants for such enzyme are evaluated and its mono-exponential behavior verified.
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Michaelis-Menten kinetics at high enzyme concentrations.高酶浓度下的米氏动力学。
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Two decades of RNA catalysis.RNA催化的二十年。
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