原子力显微镜揭示的RecA纤维的新型多态性
Novel polymorphism of RecA fibrils revealed by atomic force microscopy.
作者信息
Sattin Bernie D, Goh M Cynthia
机构信息
Department of Chemistry, University of Toronto, Toronto, ON M5S 3H6, Canada.
出版信息
J Biol Phys. 2006 Oct;32(2):153-68. doi: 10.1007/s10867-006-9010-3.
Abstract
RecA fibrils in physiological conditions have been successfully imaged using Tapping Mode atomic force microscopy. This represents the first time images of recA have been obtained without drying, freezing and/or exposure to high vacuum conditions. While previously observed structures - the monomer, the hexamer, the short rod - were seen, a new type of fibril was also observed. This protofibril is narrower in diameter than the standard fibril, and occurs in three distinct morphologies: aperiodic, 100-nm periodic, and 150-nm periodic. In addition, much longer rods were observed, and appear curved and even circular.
摘要
利用轻敲模式原子力显微镜已成功对生理条件下的RecA纤维进行成像。这是首次在不进行干燥、冷冻和/或暴露于高真空条件的情况下获得RecA的图像。虽然之前观察到的结构——单体、六聚体、短杆——都能看到，但也观察到了一种新型纤维。这种原纤维的直径比标准纤维窄，并且呈现出三种不同的形态：无周期的、100纳米周期的和150纳米周期的。此外，还观察到长得多的杆，并且看起来是弯曲的甚至是圆形的。
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