通过集成的湿磨和碱预处理来增强玉米秸秆的酶水解。
Enhancing the enzymatic hydrolysis of corn stover by an integrated wet-milling and alkali pretreatment.
机构信息
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industrial Technology, Nanjing University of Technology, Nanjing, Jiangsu, 210009, China.
出版信息
Appl Biochem Biotechnol. 2010 Apr;160(8):2449-57. doi: 10.1007/s12010-009-8736-3. Epub 2009 Aug 11.
An integrated wet-milling and alkali pretreatment was applied to corn stover prior to enzymatic hydrolysis. The effects of NaOH concentration in the pretreatment on crystalline structure, chemical composition, and reducing-sugar yield of corn stover were investigated, and the mechanism of increasing reducing-sugar yield by the pretreatment was discussed. The experimental results showed that the crystalline structure of corn stover was disrupted, and lignin was removed, while cellulose and hemicellulose were retained in corn stover by the pretreatment with 1% NaOH in 1 h. The reducing-sugar yield from the pretreated corn stovers increased from 20.2% to 46.7% when the NaOH concentration increased from 0% to 1%. The 1% NaOH pretreated corn stover had a holocellulose conversion of 55.1%. The increase in reducing-sugar yield was related to the crystalline structure disruption and delignification of corn stover. It was clarified that the pretreatment significantly enhanced the conversion of cellulose and hemicellulose in the corn stover to sugars.
采用湿磨和碱预处理相结合的方法对玉米秸秆进行预处理，然后进行酶水解。考察了预处理过程中 NaOH 浓度对玉米秸秆结晶结构、化学组成和还原糖产率的影响，并探讨了预处理提高还原糖产率的机理。实验结果表明，1%NaOH 在 1 h 内预处理可以破坏玉米秸秆的结晶结构，去除木质素，同时保留纤维素和半纤维素。当 NaOH 浓度从 0%增加到 1%时，预处理后的玉米秸秆的还原糖产率从 20.2%增加到 46.7%。1%NaOH 预处理的玉米秸秆的全纤维素转化率为 55.1%。还原糖产率的增加与玉米秸秆结晶结构的破坏和脱木质素有关。研究结果表明，预处理可显著提高玉米秸秆中纤维素和半纤维素向糖的转化率。
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