基于联合预处理和分批进料工艺从玉米秸秆中生产高浓度糖。
High-concentration sugars production from corn stover based on combined pretreatments and fed-batch process.
机构信息
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
出版信息
Bioresour Technol. 2010 Jul;101(13):4884-8. doi: 10.1016/j.biortech.2009.12.013. Epub 2010 Jan 12.
In this paper, high-concentration sugars were produced from pretreated corn stover. The raw corn stover was pretreated in a process combining steam explosion and alkaline hydrogen-peroxide. The hemicellulose and lignin were removed greatly. The cellulose content increased to 73.2%. Fed-batch enzymatic hydrolysis was initiated with 12% (w/v) solids loading and 20 FPU/g solids. Then, 6% solids were fed consecutively at 12, 36 and 60 h. After 144 h, the final concentrations of reducing sugar, glucose, cellobiose and xylose reached 220, 175, 22 and 20 g/L, respectively. The final total biomass conversion was 60% in fed-batch process.
本文从预处理的玉米秸秆中生产高浓度糖。原料玉米秸秆经蒸汽爆破与碱性过氧化氢联合预处理，极大地去除了半纤维素和木质素。纤维素含量增加到 73.2%。采用 12%（w/v）固含物负荷和 20 FPU/g 固含物的分批补料酶水解法起始，在 12、36 和 60 h 时分别连续补料 6%的固含物。144 h 后，还原糖、葡萄糖、纤维二糖和木糖的最终浓度分别达到 220、175、22 和 20 g/L。分批补料过程的最终总生物质转化率为 60%。
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