Luo Rong, Kong Weijia, Huang Xiang, Zheng Jin'e
Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2009 May;23(10):436-8, 442.
To investigate the characteristics of T lymphocytic subsets in chronic tonsillitis patients for evaluation of their clinical implication.
Fresh peripheral blood samples were obtained from 54 chronic tonsillitis patients and 52 healthy counterparts. CD4+ and CD8+ T lymphocyte subsets including the naive (CD45RA+), memory (CD45RO+), functional (CD28+), activated (HLA-DR+, CD25+) and apoptosis (CD95+) T lymphocytes, were analyzed by flow cytometry, respectively. The clinical data such as serum Cystatin C con centration, ASO and ESR were simultaneously recorded from each chronic tonsillitis patient.
The CD4+ T cells, the rate of CD4+/CD8+ and CD4+ CD45RA+ /CD4+ CD45RO+, the CD4+ CD45RA+ T cells and the CD4+ CD25+ T cells in chronic tonsillitis were significantly lower than those of the control group (P < 0.05) while an obviously increasing percentage of memory (CD45RO+) and apoptosis (CD95+) T lymphocytes in chronic tonsillitis patients, and there were significant differences between the patients with chronic tonsillitis and the healthy volunteers (P < 0.05). Furthermore, in the chronic tonsillitis patients, Serum Cystatin C level was negatively correlated with CD4+ CD45RA+ T(P < 0.05), and not significantly correlated with other T lymphocyte subtypes (P > 0.05).
Immune disorder is present in the peripheral blood of chronic tonsillitis patients. Our data may provide valuable information for evaluation of disease progression of chronic tonsillitis patients.
研究慢性扁桃体炎患者T淋巴细胞亚群的特征，以评估其临床意义。
采集54例慢性扁桃体炎患者和52例健康对照者的新鲜外周血样本。分别采用流式细胞术分析CD4⁺和CD8⁺T淋巴细胞亚群，包括初始型（CD45RA⁺）、记忆型（CD45RO⁺）、功能型（CD28⁺）、活化型（HLA-DR⁺、CD25⁺）和凋亡型（CD95⁺）T淋巴细胞。同时记录每位慢性扁桃体炎患者的血清胱抑素C浓度、抗链球菌溶血素O（ASO）和红细胞沉降率（ESR）等临床数据。
慢性扁桃体炎患者的CD4⁺T细胞、CD4⁺/CD8⁺比值、CD4⁺CD45RA⁺/CD4⁺CD45RO⁺、CD4⁺CD45RA⁺T细胞和CD4⁺CD25⁺T细胞均显著低于对照组（P<0.05），而慢性扁桃体炎患者中记忆型（CD45RO⁺）和凋亡型（CD95⁺）T淋巴细胞的百分比明显增加，慢性扁桃体炎患者与健康志愿者之间存在显著差异（P<0.05）。此外，在慢性扁桃体炎患者中，血清胱抑素C水平与CD4⁺CD45RA⁺T细胞呈负相关（P<0.05），与其他T淋巴细胞亚群无显著相关性（P>0.05）。
慢性扁桃体炎患者外周血存在免疫紊乱。我们的数据可为评估慢性扁桃体炎患者的疾病进展提供有价值的信息。
