Jiang H, Takahara S, Kokado Y, Ishibashi M, Sonoda T
Department of Urology, Osaka University Medical School, Japan.
J Clin Lab Immunol. 1990 Oct;33(2):75-81.
The immunosuppressive mechanism of Deoxymethylspergualin (MeDSG) on human lymphocyte responses in vitro was assessed in comparison with Cyclosporin A (CyA). Peripheral blood mononuclear cells from normal human volunteers were used for assay of mixed lymphocyte reaction (MLR), cell-mediated lympholysis (CML) and blastogenesis by PHA and OKT3. MeDSG suppressed only allogenic stimulation (MLR and CML), however, CyA showed a suppressive effect for all assays. Kinetic study of MLR showed that the suppressive activity did not decrease even when MeDSG was added at day 3 or day 4. CyA, however, showed a weak suppressive effect when added at a later phase of MLR. The results suggest that MeDSG is a useful immunosuppressive agent and, in vitro, its immunosuppressive mechanism on human lymphocyte responses is quite different from CyA.
与环孢素A（CyA）相比，评估了去甲基精胍菌素（MeDSG）对体外人淋巴细胞反应的免疫抑制机制。使用来自正常人类志愿者的外周血单个核细胞进行混合淋巴细胞反应（MLR）、细胞介导的淋巴细胞溶解（CML）以及PHA和OKT3诱导的细胞增殖试验。MeDSG仅抑制同种异体刺激（MLR和CML），然而，CyA对所有试验均显示出抑制作用。MLR的动力学研究表明，即使在第3天或第4天添加MeDSG，其抑制活性也不会降低。然而，在MLR后期添加CyA时，其抑制作用较弱。结果表明，MeDSG是一种有用的免疫抑制剂，并且在体外，其对人淋巴细胞反应的免疫抑制机制与CyA有很大不同。
