Moulin B, Morin J P, Seurin-Toutain P, Borghi H, Fillastre J P
Institut National de la Santé et de la Recherche Médicale I.N.S.E.R.M. U-295, University of Rouen, France.
Int J Tissue React. 1990;12(5):309-17.
The role of the renin angiotensin system (RAS) during pregnancy is not fully understood but numerous studies point to its importance in the homoeostasis of the fetal blood pressure and in the physiology of the fetal kidney near term. The aim of this study was to investigate the tissue distribution of angiotensin-converting enzyme (ACE) in the pregnant rabbit and its fetus and to assess the effect of Perindopril (PIL) a new ACE inhibitor on maternal and fetal tissue ACE activity. On day 28 of gestation, animals of the experimental groups were gavage-fed with 1 mg PIL or 10 mg PIL. ACE activity was assayed in tissue homogenates and serum with a radio-enzymatic method using (Gly-1-14C)-hippuryl-L-histidyl-leucine as specific substrate. In the kidney of control pregnant rabbit, decreasing values of ACE were found with a concentration gradient from the cortex to the inner papilla. ACE values in lung were comparable to those seen in kidney cortex. A significant effect of PIL was found with a percentage of inhibition of ACE activity in the renal cortex above 74% after 1 mg PIL and 88% after 10 mg PIL. In the control group, ACE activity was predominant in lung of fetuses. After maternal administration of 1 mg PIL, ACE activity fell significantly in fetal serum, placenta and fetal lung, but not in fetal kidney. Ten mg PIL produced a further significant decrease in ACE activity in fetal organs and serum. Plasma renin activity (PRA) was significantly stimulated after PIL administration in both mothers and fetuses.(ABSTRACT TRUNCATED AT 250 WORDS)
肾素血管紧张素系统（RAS）在孕期的作用尚未完全明确，但大量研究表明其在胎儿血压稳态及足月时胎儿肾脏生理功能中具有重要意义。本研究旨在探究血管紧张素转换酶（ACE）在妊娠兔及其胎儿体内的组织分布，并评估新型ACE抑制剂培哚普利（PIL）对母体和胎儿组织ACE活性的影响。妊娠第28天，实验组动物分别经口灌胃给予1 mg PIL或10 mg PIL。采用放射性酶法，以（甘氨酸-1-14C）-马尿酰-L-组氨酰-亮氨酸作为特异性底物，测定组织匀浆和血清中的ACE活性。在对照妊娠兔的肾脏中，ACE值从皮质到内乳头呈浓度梯度降低。肺中的ACE值与肾皮质中的相当。发现PIL具有显著作用，1 mg PIL后肾皮质中ACE活性的抑制率超过74%，10 mg PIL后为88%。在对照组中，ACE活性在胎儿肺中占主导。母体给予1 mg PIL后，胎儿血清、胎盘和胎儿肺中的ACE活性显著下降，但胎儿肾脏中未下降。10 mg PIL使胎儿器官和血清中的ACE活性进一步显著降低。给予PIL后，母体和胎儿的血浆肾素活性（PRA）均受到显著刺激。（摘要截取自250字）
