Department of Radiology, Research Institute of Radiological Science, Yonsei University College of Medicine, Seoul, Korea.
Head Neck. 2010 Apr;32(4):490-8. doi: 10.1002/hed.21210.
To evaluate the diagnostic value of dual priming oligonucleotide (DPO)-based multiplex polymerase chain reaction (PCR) for the detection of BRAF(V600E) mutations in ultrasound-guided fine-needle aspiration biopsy (US-FNAB) of thyroid nodules.
Our institutional review board approved this retrospective study, and informed consent was not required from patients. The 130 patients underwent US-FNAB to evaluate BRAF status in thyroid nodules. In FNAB washouts, DPO-based multiplex PCR, direct DNA sequencing, and PCR-restriction fragment length polymorphism (RFLP) were used to detect BRAF(V600E). The diagnostic performance of these methods was calculated. We compared cytologic results by BRAF status.
Diagnostic accuracy and sensitivity were highest when screening with DPO-based multiplex PCR. BRAF(V600E) positivity was a useful marker at thyroid nodules with "suspicious for papillary thyroid carcinoma" or "inadequate" cytological result.
DPO-based multiplex PCR may be an alternative to direct DNA sequencing because of its high sensitivity, high accuracy, and simplicity. BRAF(V600E) may be a useful additional diagnostic marker in BRAF(V600E)-prevalent areas.
评估基于双重引物寡核苷酸（DPO）的多重聚合酶链反应（PCR）在超声引导下细针抽吸活检（US-FNAB）甲状腺结节中 BRAF(V600E)突变检测中的诊断价值。
我们的机构审查委员会批准了这项回顾性研究，患者不需要签署知情同意书。130 名患者接受了 US-FNAB 以评估甲状腺结节中的 BRAF 状态。在 FNAB 冲洗液中，使用 DPO 基于多重 PCR、直接 DNA 测序和 PCR-限制性片段长度多态性（RFLP）检测 BRAF(V600E)。计算这些方法的诊断性能。我们比较了基于 BRAF 状态的细胞学结果。
当使用 DPO 基于多重 PCR 进行筛查时，诊断准确性和敏感性最高。在具有“疑似甲状腺乳头状癌”或“不充分”细胞学结果的甲状腺结节中，BRAF(V600E)阳性是一个有用的标志物。
由于其高灵敏度、高准确性和简单性，DPO 基于多重 PCR 可能是直接 DNA 测序的替代方法。在 BRAF(V600E)流行地区，BRAF(V600E)可能是一个有用的附加诊断标志物。
