Boettiger D, Roby K, Brumbaugh J, Biehl J, Holtzer H
Cell. 1977 Aug;11(4):881-90. doi: 10.1016/0092-8674(77)90299-9.
Retinal melanoblasts were transformed by a temperature-sensitive mutant of Rous sarcoma virus (ts-RSV). At the permissive temperature for transformation, the cells cease melanin synthesis, degrade their melanosomes and release much of their accumulated melanin into the medium. At the nonpermissive temperature, the cells assume an epithelioid morphology, actively synthesize melanin and become difficult to distinguish from normal uninfected control cultures. Both the transformed phenotype and the differentiated cell phenotype are temperature-dependent. Infected retinal melanoblasts which are incubated at the nonpermissive temperature and which accumulate a large amount of melanin are unable to transform in response to a temperature shift; instead, the cells degenerate and die. Retinal melanoblasts can be infected by subgroups A, B, C and D of RSV; however, their level of susceptibility to infection is about 1/40 compared to fibroblasts. Cultures infected by ts-RSV produce virus at both temperatures, suggesting that cell phenotype does not regulate virus synthesis.
视网膜成黑素细胞被劳氏肉瘤病毒的温度敏感突变体（ts-RSV）转化。在允许转化的温度下，细胞停止黑色素合成，降解其黑素小体，并将大量积累的黑色素释放到培养基中。在非允许温度下，细胞呈现上皮样形态，积极合成黑色素，变得难以与正常未感染的对照培养物区分开来。转化表型和分化细胞表型均依赖于温度。在非允许温度下孵育并积累大量黑色素的受感染视网膜成黑素细胞，在温度变化时无法转化；相反，细胞会退化并死亡。视网膜成黑素细胞可被RSV的A、B、C和D亚组感染；然而，与成纤维细胞相比，它们的感染易感性水平约为1/40。受ts-RSV感染的培养物在两种温度下都会产生病毒，这表明细胞表型不调节病毒合成。
