Anthony D T, Schuetze S M, Rubin L L
Proc Natl Acad Sci U S A. 1984 Apr;81(7):2265-9. doi: 10.1073/pnas.81.7.2265.
Acetylcholine receptors aggregate in the membrane of cultured chicken myotubes; the process of receptor clustering can be stimulated by exogenous factors that we, among others, have begun to characterize. Chicken myoblasts transformed by temperature-sensitive mutants of Rous sarcoma virus, such as tsNY68, fuse to form multinucleated myotubes at 42 degrees C, the nonpermissive temperature for transformation. However, tsNY68-infected myotubes do not cluster acetylcholine receptors at 42 degrees C, even in the presence of active clustering agents. This phenomenon is not merely a result of viral infection, since myotubes infected with a transformation-deficient viral mutant, td107A, behave like noninfected myotubes with respect to receptor clustering; thus, the effects of tsNY68 on the clustering process must be mediated by the src gene product. These experiments may provide a method of identifying essential elements of acetylcholine receptor clusters.
乙酰胆碱受体聚集在培养的鸡肌管膜中；受体聚集过程可被我们等已开始进行特性描述的外源性因素所刺激。被劳氏肉瘤病毒温度敏感突变体（如tsNY68）转化的鸡成肌细胞，在42℃（转化的非允许温度）时融合形成多核肌管。然而，即使存在活性聚集剂，被tsNY68感染的肌管在42℃时也不会聚集乙酰胆碱受体。这种现象不仅仅是病毒感染的结果，因为感染了转化缺陷病毒突变体td107A的肌管在受体聚集方面的表现与未感染的肌管相似；因此，tsNY68对聚集过程的影响必定是由src基因产物介导的。这些实验可能提供一种鉴定乙酰胆碱受体簇关键成分的方法。
