Loshon C A, McComb R B, Bond L W, Bowers G N, Coleman W H, Gwynn R H
Clin Chem. 1977 Sep;23(9):1576-80.
We describe some characteristics of the mode of formation of inhibitors of lactate dehydrogenase from commercial NADH. Inhibitor formation is time- and concentration-dependent and also varies with the commercial source of NADH. At least two inhibitory components can form in concentrated NADH solutions. One of these can be separated from NADH by chromatography on either diethylaminoethyl-celluose or diethylaminoethyl-Sephadex; the second cannot. The NADH-associated inhibitor appeared to be present in each of the three commercial NADH preparations studied. The 260 nm/340 nm absorbance ratio was of no help in locating this inhibitor during chromatography.
我们描述了由市售烟酰胺腺嘌呤二核苷酸（NADH）形成乳酸脱氢酶抑制剂的形成模式的一些特征。抑制剂的形成与时间和浓度有关，并且也因NADH的商业来源而异。在浓缩的NADH溶液中至少可以形成两种抑制成分。其中一种可以通过在二乙氨基乙基纤维素或二乙氨基乙基葡聚糖凝胶上进行色谱分离与NADH分离；第二种则不能。与NADH相关的抑制剂似乎在所研究的三种市售NADH制剂中均存在。在色谱分析过程中，260nm/340nm吸光度比对于定位这种抑制剂没有帮助。
