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β-肾上腺素能激动剂对发育中大鼠下颌下腺胱抑素S基因的表达及诱导作用

Expression and induction by beta-adrenergic agonists of the cystatin S gene in submandibular glands of developing rats.

作者信息

Shaw P A, Barka T, Woodin A, Schacter B S, Cox J L

机构信息

Department of Cell Biology, Mount Sinai School of Medicine, New York, NY 10029.

出版信息

Biochem J. 1990 Jan 1;265(1):115-20. doi: 10.1042/bj2650115.

DOI:10.1042/bj2650115
PMID:1967932
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1136621/
Abstract

Repeated administration of the beta-adrenergic agonist isoprenaline (isoproterenol, IPR), which produces hypertrophic/hyperplastic enlargements of rat submandibular and parotid glands, induces synthesis of a secretory protein shown to be a cysteine proteinase inhibitor, rat cystatin S. In the current study, Northern blot and hybridizations in situ were carried out to establish the developmental and beta-adrenergic regulation of the expression of the cystatin S gene. Cystatin S mRNA was not detected in submandibular glands of 20-day-old fetuses, nor in the glands of newborn or 10-day-old rats. However, steady-state levels of cystatin S mRNA increased between 21 and 28 days, reaching a conspicuously high concentration at 28 days; cystatin S mRNA then declined rapidly to a barely detectable level in glands of 32-day-old rats. IPR administration for 4 days induced high levels of cystatin S mRNA in submandibular glands of developing and adult rats. In both prepubertal and mature animals, induction of cystatin S mRNA in submandibular glands was more pronounced in female than in male animals. Hybridizations in situ revealed cystatin S mRNA only in acinar but not in duct cells of the submandibular gland. Developmentally, expression of the cystatin S gene coincided with acinar cell differentiation. These data suggest a complex neural, hormonal and developmental regulation of salivary cystatin genes.

摘要

反复给予β-肾上腺素能激动剂异丙肾上腺素（isoproterenol，IPR）可使大鼠颌下腺和腮腺发生肥大/增生性肿大，该激动剂还能诱导一种分泌蛋白的合成，该蛋白被证明是一种半胱氨酸蛋白酶抑制剂，即大鼠胱抑素S。在本研究中，进行了Northern印迹和原位杂交，以确定胱抑素S基因表达的发育调控和β-肾上腺素能调控。在20日龄胎儿的颌下腺中未检测到胱抑素S mRNA，在新生大鼠或10日龄大鼠的腺体中也未检测到。然而，胱抑素S mRNA的稳态水平在21至28天之间升高，在28天时达到明显高浓度；然后胱抑素S mRNA迅速下降至32日龄大鼠腺体中几乎检测不到的水平。给予IPR 4天可诱导发育中和成年大鼠颌下腺中高水平的胱抑素S mRNA。在青春期前和成年动物中，颌下腺中胱抑素S mRNA的诱导在雌性动物中比在雄性动物中更明显。原位杂交显示胱抑素S mRNA仅存在于颌下腺的腺泡细胞中，而不存在于导管细胞中。从发育角度来看，胱抑素S基因的表达与腺泡细胞分化同步。这些数据表明唾液胱抑素基因存在复杂的神经、激素和发育调控。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1277/1136621/d3b67a86b69c/biochemj00192-0125-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1277/1136621/2578cf126a4c/biochemj00192-0122-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1277/1136621/712c6bb9ff48/biochemj00192-0123-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1277/1136621/40e68f345832/biochemj00192-0124-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1277/1136621/c5703d45d578/biochemj00192-0124-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1277/1136621/d3b67a86b69c/biochemj00192-0125-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1277/1136621/2578cf126a4c/biochemj00192-0122-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1277/1136621/712c6bb9ff48/biochemj00192-0123-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1277/1136621/40e68f345832/biochemj00192-0124-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1277/1136621/c5703d45d578/biochemj00192-0124-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1277/1136621/d3b67a86b69c/biochemj00192-0125-a.jpg

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