Institute of Genomics and Integrative Biology, CSIR, Mall Road, Delhi 110 007, India.
Langmuir. 2009 Dec 15;25(24):13770-5. doi: 10.1021/la901546t.
The interaction between DNA and amino-acid-based surfactants with different linker groups was investigated by gel electrophoresis, ethidium bromide exclusion assays, circular dichroism, and melting temperature determinations. The studies showed that the strength of the interaction between the oligonucleotides and the surfactants is highly dependent on the linker of the surfactant. For ester surfactants, no significant interaction was observed for surfactant-to-DNA charge ratios up to 12. On the other hand, amide surfactants were shown to interact strongly with the oligonucleotides; these surfactants could displace up to 75% of the ethidium bromide molecules bound to the DNA and induced significant changes in the circular dichroism spectra. When comparing the headgroups of the surfactants, it was observed that surfactants with more hydrophobic headgroups (proline vs alanine) interacted more strongly with the DNA, in good agreement with previous studies.
通过凝胶电泳、溴化乙锭排除试验、圆二色性和熔点测定研究了具有不同连接基团的 DNA 和氨基酸基表面活性剂之间的相互作用。研究表明，寡核苷酸与表面活性剂之间的相互作用强度高度依赖于表面活性剂的连接基团。对于酯表面活性剂，在表面活性剂与 DNA 的电荷比高达 12 时，没有观察到明显的相互作用。另一方面，酰胺表面活性剂被证明与寡核苷酸强烈相互作用；这些表面活性剂可以置换多达 75%与 DNA 结合的溴化乙锭分子，并引起圆二色性光谱的显著变化。当比较表面活性剂的头基时，观察到具有疏水头基（脯氨酸与丙氨酸）的表面活性剂与 DNA 的相互作用更强，这与先前的研究结果一致。
