Department of Microbiology, Karolinska Institute, Box 280, 17177 Stockholm, Sweden.
Microbes Infect. 2009 Dec;11(14-15):1114-21. doi: 10.1016/j.micinf.2009.08.005. Epub 2009 Aug 12.
Monitoring the spread of mycobacterium in vivo using biophotonic imaging provides a fast, reliable and sensitive method to evaluate the distribution of the infection. Moreover, this technique allows for a significant reduction in the number of animals required in comparison to conventional anatomopathological studies. Here, we describe for the first time and validate the use of a luciferase-tagged recombinant Mycobacterium bovis BCG for non-invasive bioluminescent imaging of 1) bacterial dissemination in tissues, 2) the efficacy of treatment with anti-mycobacterial drugs and 3) the role of adaptive immune responses in controlling mycobacterial infection in vivo.
利用生物发光成像技术监测体内分枝杆菌的传播提供了一种快速、可靠和敏感的方法来评估感染的分布。此外，与传统的解剖病理学研究相比，该技术可以显著减少所需动物的数量。在这里，我们首次描述并验证了使用荧光素酶标记的重组牛分枝杆菌卡介苗进行非侵入性生物发光成像的用途，用于 1）细菌在组织中的传播，2）抗分枝杆菌药物治疗的效果，以及 3）适应性免疫反应在控制体内分枝杆菌感染中的作用。
