Delelis Olivier, Zamborlini Alessia, Thierry Sylvain, Saïb Ali
LBPA, CNRS UMR8113, Ecole Normale Supérieure de Cachan, 61 Avenue du Président Wilson, 94235 Cachan Cedex, France.
Biochim Biophys Acta. 2010 Mar-Apr;1799(3-4):207-16. doi: 10.1016/j.bbagrm.2009.08.005. Epub 2009 Aug 13.
Since integration into the host cell genome is an obligatory step for their replication, retro-elements are both potent insertional mutagens and also suitable vectors for gene therapy. Many recent studies reported that the integration process is not random but, on the contrary, higly regulated at the molecular level. Many viral proteins and cellular factors play a key role in the integration step, explaining the reason why different retro-elements display distinct integration profiles. This review describes the recent highlights about integration of retro-elements with particular focus on the mechanisms underlying the specificity of integration target-site selection and the step of chromosomal tethering which preceeds insertion of the provirus.
由于整合到宿主细胞基因组是逆转录元件复制的必经步骤，因此它们既是强大的插入诱变剂，也是适合基因治疗的载体。最近许多研究报告称，整合过程并非随机发生，相反，在分子水平上受到高度调控。许多病毒蛋白和细胞因子在整合步骤中起关键作用，这解释了不同逆转录元件为何呈现出不同的整合模式。本综述描述了逆转录元件整合的最新要点，特别关注整合靶位点选择特异性的潜在机制以及前病毒插入之前的染色体拴系步骤。
