Mine Atsushi, De Munck Jan, Cardoso Marcio V, Van Landuyt Kirsten L, Poitevin André, Kuboki Takuo, Yoshida Yasuhiro, Suzuki Kazuomi, Lambrechts Paul, Van Meerbeek Bart
Leuven BIOMAT Research Cluster, Department of Conservative Dentistry, School of Dentistry, Oral Pathology and Maxillo-Facial Surgery, Catholic University of Leuven, Leuven, Belgium.
J Dent. 2009 Nov;37(11):872-83. doi: 10.1016/j.jdent.2009.06.020. Epub 2009 Jul 7.
Among contemporary adhesives, self-etch adhesives have been adopted by general practitioners for routine adhesive restorative purposes, mainly because of their ease of use. However, many versions that differ for their clinical application procedure, pH, number of components, etc., are currently available on the market. The purpose of this study was to determine the bonding effectiveness of two new self-etch adhesives (Adper Easy Bond and Adper ScotchBond SE, 3M ESPE) to enamel and dentin using a micro-tensile bond strength (microTBS) protocol and to characterise the interfacial ultra-structure at enamel and dentin using transmission electron microscopy (TEM).
The adhesives were applied onto coronal human enamel and dentin surfaces and built up with the micro-hybrid resin composite Z100 (3M ESPE). The 'gold-standard' two-step self-etch adhesive Clearfil SE Bond (Kuraray) served as control. Specimens were sectioned to sticks and trimmed at the interface to a cylindrical hour-glass shape ('trimmed' micro-specimens). Non-demineralized and demineralized TEM sections through the adhesive-dentin/enamel interface were prepared by ultra-microtomy.
The microTBS of the two self-etch adhesives to enamel was statistically significantly lower than that of the control. To dentin, the microTBS of Adper Easy Bond was significantly lower than that of Adper ScotchBond SE and the control. TEM showed a tight interface to enamel for all three self-etch adhesives. A relatively thick, completely demineralized and acid-resistant hybrid layer was formed at dentin by Adper ScotchBond SE, whereas the interaction of Adper Easy Bond was much shallower, and comparable to that of so-called 'ultra-mild' self-etch adhesives. Some degree of spot- and cluster-like nano-leakage was observed for both adhesives, but did not differ in extent and form from that observed for the control.
Although the new two self-etch adhesives revealed a tight interaction at both enamel and dentin, their bond strength to both tooth tissues was generally lower than that of the control adhesive. Nevertheless, their bonding effectiveness appears in line with other simplified self-etch adhesives.
在当代粘合剂中，自酸蚀粘合剂已被全科医生用于常规粘合剂修复目的，主要是因为其使用方便。然而，目前市场上有许多版本，它们在临床应用程序、pH值、成分数量等方面存在差异。本研究的目的是使用微拉伸粘结强度（microTBS）方案确定两种新型自酸蚀粘合剂（Adper Easy Bond和Adper ScotchBond SE，3M ESPE）对牙釉质和牙本质的粘结效果，并使用透射电子显微镜（TEM）对牙釉质和牙本质的界面超微结构进行表征。
将粘合剂应用于人类牙冠的牙釉质和牙本质表面，并用微混合树脂复合材料Z100（3M ESPE）进行堆积。“金标准”两步自酸蚀粘合剂Clearfil SE Bond（可乐丽）用作对照。将标本切成小棒，并在界面处修剪成圆柱形沙漏形状（“修剪”后的微标本）。通过超薄切片术制备穿过粘合剂-牙本质/牙釉质界面的未脱矿和脱矿的TEM切片。
两种自酸蚀粘合剂对牙釉质的microTBS在统计学上显著低于对照。对于牙本质，Adper Easy Bond的microTBS显著低于Adper ScotchBond SE和对照。TEM显示所有三种自酸蚀粘合剂与牙釉质的界面紧密。Adper ScotchBond SE在牙本质上形成了相对较厚、完全脱矿且耐酸的混合层，而Adper Easy Bond的相互作用则浅得多，与所谓的“超温和”自酸蚀粘合剂相当。两种粘合剂均观察到一定程度的点状和簇状纳米渗漏，但在程度和形式上与对照观察到的无差异。
尽管这两种新型自酸蚀粘合剂在牙釉质和牙本质上均显示出紧密的相互作用，但它们与两种牙齿组织的粘结强度通常低于对照粘合剂。然而，它们的粘结效果似乎与其他简化的自酸蚀粘合剂一致。
