Department of Gastroenterology, Faculty of Medicine, Cukurova University, 01330 Adana, Turkey.
Cancer Epidemiol. 2009 Oct;33(3-4):261-4. doi: 10.1016/j.canep.2009.06.001. Epub 2009 Jul 2.
Tumor necrosis factor-alpha (TNF-alpha) is a pro-inflammatory cytokine that may act as an endogenous tumor promoter. A genetic polymorphism of TNF-alpha gene at position -308 promoter region is involved in the regulation of expression level and has been found to be associated with susceptibility to various types of cancer.
To determine the association of the TNF-alpha gene G-308A polymorphism on the risk of hepatocellular carcinoma (HCC) in a Turkish population, a hospital-based case-control study was designed consisting of 110 diagnosis subjects with hepatocellular carcinoma and 110 cancer-free control subjects matched on age, gender, smoking and alcohol status. The genotype frequency of this polymorphism was determined by using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay.
The distribution G-308A genotype was significantly associated with the risk of HCC (p<0.001, odds ratio [OR]=4.75, 95% confidence interval [CI]=2.25-9.82 for -308 AA/GA genotypes versus GG genotype).
We suggested that the presence of the high producer allele -308A in the TNF-alpha gene appears to be associated with an increased risk for the development of HCC in Turkish population.
肿瘤坏死因子-α（TNF-α）是一种促炎细胞因子，可能作为内源性肿瘤促进剂发挥作用。TNF-α 基因在 -308 启动子区域的遗传多态性参与表达水平的调节，并已发现与各种类型的癌症易感性有关。
为了确定 TNF-α 基因 G-308A 多态性在土耳其人群中对肝细胞癌（HCC）风险的影响，设计了一项基于医院的病例对照研究，包括 110 名诊断为肝细胞癌的患者和 110 名年龄、性别、吸烟和饮酒状况相匹配的无癌症对照组。使用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析确定该多态性的基因型频率。
G-308A 基因型的分布与 HCC 的风险显著相关（p<0.001，比值比[OR]=4.75，95%置信区间[CI]=2.25-9.82，-308 AA/GA 基因型与 GG 基因型相比）。
我们认为 TNF-α 基因中高产生等位基因-308A 的存在似乎与土耳其人群中 HCC 发生风险增加有关。
