Division of Hematology, Department of Medicine, University of Hong Kong, Hong Kong.
Leuk Res. 2010 Jan;34(1):119-22. doi: 10.1016/j.leukres.2009.07.035. Epub 2009 Aug 15.
In this study, we tested if FLT3/internal tandem duplication (ITD) in acute myeloid leukemia (AML) might occur at different hierarchical stages during leukemogenesis. In 56 AML cases, 10 showed FLT3/ITD (single ITD=5; multiple ITD=5). Myeloblasts from seven cases (CD34-selected=4; unselected=3) were transplanted into NOD/SCID mice. Five cases engrafted successfully into 14 mice. Two patients carried single FLT3/ITD subclones, which were maintained during primary and secondary transplantations. In three patients with multiple FLT3/ITD subclones, some subclones persisted or expanded while others diminished upon transplantation. Their different engraftment capabilities in NOD/SCID mice supported the proposition that FLT3/ITD might occur at different stages during leukemogenesis.
在这项研究中，我们检测了急性髓系白血病（AML）中的 FLT3/内部串联重复（ITD）是否可能在白血病发生的不同层次阶段发生。在 56 例 AML 病例中，有 10 例显示出 FLT3/ITD（单一 ITD=5；多重 ITD=5）。来自七个病例的髓样前体细胞（CD34 选择=4；未选择=3）被移植到 NOD/SCID 小鼠中。五个病例成功地植入了 14 只小鼠中。两名患者携带单一的 FLT3/ITD 亚克隆，这些亚克隆在原发性和继发性移植期间得以维持。在三个具有多个 FLT3/ITD 亚克隆的患者中，一些亚克隆在移植后持续存在或扩增，而另一些亚克隆则减少。它们在 NOD/SCID 小鼠中的不同植入能力支持了这样的假设，即 FLT3/ITD 可能在白血病发生的不同阶段发生。
