Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Ishikawa, Japan.
Bioelectrochemistry. 2010 Feb;77(2):106-13. doi: 10.1016/j.bioelechem.2009.07.002. Epub 2009 Jul 17.
To examine the amplification of the effects of low-frequency electric fields due to the junction between the cells, the amplitude of transmembrane potential P(TMP0), the time-averaged normal stress sigma(n) and the outward force density sigma(out) on the shell-phase, and the squared intensity I(ee) of electric fields in the inner and the outer phases induced by uniform external ac fields were calculated with the boundary-element method for doublet-shaped cell models consisting of two spheres of the same size connected by the junction; the results were compared with those in a spherical model. When the external fields were parallel to the long axis of the doublet-shaped models, P(TMP0), sigma(n) and sigma(out) at the pole were greater than those in the spherical model, and sigma(out) and I(ee) at the junction increased with the decrease in the junction-radius. The external fields perpendicular to the long axis caused I(ee) greater than that at the center of the spherical model and negative sigma(out), at the junction. The amplification of P(TMP0), sigma(n), sigma(out) and I(ee) took place within restricted frequency-regions that could be specified by the characteristic frequencies for the frequency-dependence of the polarization factor of the models.
为了研究细胞膜间连接导致低频电场效应放大的情况，我们采用边界元法计算了由两个相同大小的球体通过连接部构成的双锥细胞模型的跨膜电位 P(TMP0)、时间平均法向应力 sigma(n)和外向力密度 sigma(out)在壳层相位的变化，以及内外相均匀外交流场诱导的电场平方强度 I(ee)。我们将计算结果与球形模型的结果进行了比较。当外场与双锥模型的长轴平行时，极部的 P(TMP0)、sigma(n)和 sigma(out)大于球形模型的值，而 sigma(out)和连接部的 I(ee)随连接部半径的减小而增大。与长轴垂直的外场会导致连接部的 I(ee)大于球形模型中心的值，并产生负的 sigma(out)。P(TMP0)、sigma(n)、sigma(out)和 I(ee)的放大发生在可以由模型极化因子频率依赖性的特征频率指定的特定频率区域内。
