Division of Pathway Medicine, University of Edinburgh, Edinburgh, UK EH16 4SB.
Analyst. 2009 Sep;134(9):1873-9. doi: 10.1039/b900325h. Epub 2009 Jul 13.
DNA nanoswitches can be designed to detect unlabelled nucleic acid targets and have been shown to discriminate between targets which differ in the identity of only one base. This paper demonstrates that the fluorescent base analogue 2-aminopurine (AP) can be used to discriminate between nanoswitches with and without targets and to discriminate between matched and mismatched targets. In particular, we have used both steady-state and time-resolved fluorescence spectroscopy to determine differences in AP environment at the branchpoint of nanoswitches assembled using complementary targets and targets which incorporate single base mismatches.
DNA 纳米开关可用于检测未标记的核酸靶标，并且已被证明能够区分靶标，这些靶标仅在一个碱基的身份上有所不同。本文证明，荧光碱基类似物 2-氨基嘌呤 (AP) 可用于区分带有和不带有靶标的纳米开关，以及区分匹配和不匹配的靶标。特别是，我们使用稳态和时间分辨荧光光谱法来确定使用互补靶标和包含单个碱基错配的靶标组装的纳米开关分支点处的 AP 环境差异。
