Institut National de la Recherche Agronomique, Laboratoire de Biotechnologie de l'Environnement, Avenue des Etangs, Narbonne, France.
Appl Microbiol Biotechnol. 2010 Jan;85(3):691-701. doi: 10.1007/s00253-009-2160-z. Epub 2009 Aug 15.
The biodegradation of estradiol (E2), estrone (E1), and ethinylestradiol (EE2) was investigated using mixed bacterial cultures enriched from activated sludge. Enrichments were carried out on E2 or EE2 in batch conditions with acetonitrile as additional carbon source. Degradation experiments were performed both using hormones as sole carbon source or with an additional source. The hormones were completely degraded by these cultures. Estradiol was rapidly converted to E1 within 24 h. Thereafter, E1 degradation began, displaying a lag phase ranging from 3 to 4 days. Estrone depletion took from 48 h to more than 6 days, depending on the culture conditions. For EE2 degradation, when it was the sole carbon source, the lag phase and the time required for its complete removal (7 and 15 days, respectively) were shorter that in cultures with a supplementary carbon source. The specific degradation rates observed for E2 both with and without an additional carbon source were similar. By contrast, the specific degradation rates for E1 and EE2 were, respectively, seven and 20 times faster when these hormones were supplied as the sole carbon source. The bacterial community structure of each culture was characterized by molecular and cultural methods. The mixed cultures were made up of species belonging to Alcaligenes faecalis, Pusillimonas sp., Denitrobacter sp., and Brevundimonas diminuta or related to uncultured Bacteroidetes. The isolated strain B. diminuta achieved the conversion of E2 to E1.
采用从活性污泥中富集的混合细菌培养物研究了雌二醇（E2）、雌酮（E1）和乙炔基雌二醇（EE2）的生物降解。在含有乙腈作为额外碳源的条件下，通过批处理对 E2 或 EE2 进行了富集。降解实验既使用激素作为唯一碳源进行，也使用额外的碳源进行。这些培养物完全降解了这些激素。E2 在 24 小时内迅速转化为 E1。此后，E1 的降解开始，显示出 3 到 4 天的滞后期。E1 的消耗时间从 48 小时到 6 天以上，具体取决于培养条件。对于 EE2 的降解，当它是唯一的碳源时，其滞后期和完全去除所需的时间（分别为 7 天和 15 天）比在有补充碳源的培养物中要短。有无额外碳源时，E2 的特定降解率相似。相比之下，当这些激素作为唯一碳源时，E1 和 EE2 的特定降解率分别快了 7 倍和 20 倍。通过分子和文化方法对每种培养物的细菌群落结构进行了表征。混合培养物由属于粪产碱杆菌、短小普雷沃氏菌、脱氮副球菌和纤细短小杆菌或与之相关的未培养拟杆菌的物种组成。分离的短小普雷沃氏菌菌株实现了 E2 向 E1 的转化。
