使用量子点对小鼠的血管和淋巴流动进行成像。
Imaging vasculature and lymphatic flow in mice using quantum dots.
作者信息
Ballou Byron, Ernst Lauren A, Andreko Susan, Fitzpatrick James A J, Lagerholm B Christoffer, Waggoner Alan S, Bruchez Marcel P
机构信息
Molecular Biosensor and Imaging Center, Carnegie Mellon University, Pittsburgh, PA, USA.
出版信息
Methods Mol Biol. 2009;574:63-74. doi: 10.1007/978-1-60327-321-3_6.
Quantum dots are ideal probes for fluorescent imaging of vascular and lymphatic tissues. On injection into appropriate sites, red- and near-infrared-emitting quantum dots provide excellent definition of vasculature, lymphoid organs, and lymph nodes draining both normal tissues and tumors. We detail methods for use with commercially available quantum dots and discuss common difficulties.
量子点是用于血管和淋巴组织荧光成像的理想探针。将发射红色和近红外光的量子点注射到合适的部位后，能对脉管系统、淋巴器官以及引流正常组织和肿瘤的淋巴结进行清晰成像。我们详细介绍了使用市售量子点的方法，并讨论了常见的难点。
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