Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St. Lucia, Australia.
Biotechnol Bioeng. 2009 Dec 15;104(6):1240-4. doi: 10.1002/bit.22504.
We report a scalable microbioreactor architecture which uses nested dilution structures to generate a full-factorial array of cell culture conditions. The proof-of-concept microbioreactor array produces all combinations of three concentration levels of two soluble factors (3(2) = 9 unique conditions in total). The full-factorial design is especially useful in optimizing soluble factor treatments and elucidating interaction effects between factors which are otherwise difficult to deconvolute. By nesting hierarchical levels of dilution structures, and designing the device purely by resistive flow (no valves are required), suitable diffusive mixing of growth factors up to 40 kDa is achieved such that the nine culture conditions can be generated and maintained from a minimal number of stock solutions.
我们报告了一种可扩展的微生物反应器架构，该架构使用嵌套的稀释结构来生成全因子细胞培养条件的数组。该概念验证微生物反应器阵列总共产生了两种可溶性因子（3（2）= 9 个独特条件）的三种浓度水平的所有组合。全因子设计特别有助于优化可溶性因子处理并阐明因子之间的相互作用，否则这些因子很难分解。通过嵌套分层的稀释结构，并通过电阻流（无需阀）来设计设备，可实现高达 40 kDa 的生长因子的适当扩散混合，从而可以从最少数量的储备溶液中生成和维持九种培养条件。
