Li Zhiyong, Zhang Shuofeng, Chang Hongsheng, Sun Jianning, Li Fei
School of Chinese Pharmacy of Beijing University of Chinese Medicine, Beijing 100102, China.
Zhongguo Zhong Yao Za Zhi. 2009 May;34(9):1086-9.
To study the correlation between content changes of Diester diterpenoid alkaloids (DDAs) content and safety of the processed Fuzi.
Sequential and Bliss methods were used to evaluate the safety of 7 kinds of Fuzi processed with different processing time. The relationship between ED50, TD50, TI and content changes of DDAs of those processed Fuzi was studied, the correlation between the content changes and effect of different processed Fuzi was analyzed, and the toxicity of those processed Fuzi with multiple linear regression was tested.
Fuzi with good efficiency and safety contains proper hypaconitine (HA) and mesaconitine (MA). Aconitine (AC) interfered efficacy of Fuzi (negative correlation), HA showed positive correlation with toxicity and efficacy of Fuzi.
HA and MA kept in determinate proportion are very important for the safety and effectivity of processed Fuzi.
研究炮制附子中双酯型二萜生物碱（DDAs）含量变化与安全性的相关性。
采用序贯法和 Bliss 法评价 7 种不同炮制时间的附子的安全性。研究这些炮制附子的半数有效量（ED50）、半数中毒量（TD50）、治疗指数（TI）与 DDAs 含量变化的关系，分析不同炮制附子含量变化与药效的相关性，并用多元线性回归检验这些炮制附子的毒性。
药效和安全性良好的附子含有适量的次乌头碱（HA）和中乌头碱（MA）。乌头碱（AC）干扰附子药效（负相关），HA 与附子毒性和药效呈正相关。
HA 和 MA 保持一定比例对炮制附子的安全性和有效性非常重要。
