[白芷根中的酪氨酸酶抑制剂]

[Tyrosinase inhibitor from Angelica dahurica roots].

作者信息

Piao Xianglan

机构信息

Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2009 May;34(9):1117-20.

PMID:19685748

Abstract

OBJECTIVE

To isolate and identify the tyrosinase inhibitory constituent from Angelica dahurica.

METHOD

A tyrosinase inhibitory constituent was isolated by silica gel column chromatography combined with a bioassay-linked HPLC method.

RESULT

An extract of heat-processed A. dahurica roots showed more potent tyrosinase inhibitory activity than that of the crude drug. Tyrosinase inhibitory furanocoumarin, 8-hydroxy-5-methoxypsoralen,was isolated. It express stronger tyrosinase inhibition than the known tyrosinase inhibitor, kojic acid (IC50 = 0.0907 mmol x L(-1)) with IC50 value of 0.0086 mmol x L(-1). Therefore, it is contributive to the increased tyrosinase inhibitory activity of the heat-processed A. dahurica.

CONCLUSION

The active constituent was 8-hydroxy-5-methoxypsoralen from the heat-processed A. dahurica.

摘要

目的

从白芷中分离并鉴定酪氨酸酶抑制成分。

方法

采用硅胶柱色谱结合生物活性导向的高效液相色谱法分离酪氨酸酶抑制成分。

结果

白芷根的炮制提取物比生药表现出更强的酪氨酸酶抑制活性。分离得到酪氨酸酶抑制性呋喃香豆素8-羟基-5-甲氧基补骨脂素。它比已知的酪氨酸酶抑制剂曲酸（IC50 = 0.0907 mmol·L⁻¹）表现出更强的酪氨酸酶抑制作用，IC50值为0.0086 mmol·L⁻¹。因此，它有助于白芷炮制后酪氨酸酶抑制活性的提高。