Tohoku University Graduate School of Dentistry, Sendai, Japan.
Tissue Eng Part C Methods. 2010 Jun;16(3):471-8. doi: 10.1089/ten.TEC.2009.0212.
The present study was designed to investigate whether mechanical testing in conjunction with microcomputed tomography (microCT) analysis can be used to evaluate the quality of regenerated bone enhanced by the implantation of a composite composed of granular octacalcium phosphate and collagen (OCP/Col) matrix. Previous studies confirmed that the granules of OCP alone or OCP in Col matrix tend to mature into bone-like hydroxyapatite and enhance bone regeneration coupled with its own biodegradation, if implanted in various bony sites. OCP/Col was implanted in rat calvaria critical-sized bone defect for 4 to 12 weeks for microindentation, mechanical testing, microCT imaging, and histological examinations. The microindentation testing of the regenerated bone revealed a progressive increase of the Vickers hardness showing the highest value in 12 weeks. The Vickers hardness was in good agreement with both the parameters of microCT and the mechanical property; the quality of regenerated bone increased progressively with the implantation periods. The regenerated bone exhibited a mature bone-like matrix structure with osteocytes histologically. The quality of the regenerated bone was compatible to that of normal control calvaria bone regarding the mechanical properties. The results indicate that microindentation testing can be used to evaluate the quality of regenerated bone in the initial regeneration and the development of bone enhanced by OCP granules within Col matrix. It appears that the implantation of OCP/Col could be a model study to determine the quality of the regenerated bone.
本研究旨在探讨机械测试结合微计算机断层扫描（microCT）分析是否可用于评估植入颗粒状八钙磷酸盐和胶原蛋白（OCP/Col）基质复合材料增强再生骨的质量。先前的研究证实，单独的 OCP 颗粒或 OCP 在 Col 基质中倾向于成熟为类骨羟基磷灰石，并在自身生物降解的情况下增强骨再生，如果植入各种骨部位。将 OCP/Col 植入大鼠颅骨临界尺寸骨缺损中 4 至 12 周，进行微压痕、机械测试、microCT 成像和组织学检查。再生骨的微压痕测试显示维氏硬度逐渐增加，在 12 周时达到最高值。维氏硬度与 microCT 和机械性能的参数非常吻合；随着植入时间的延长，再生骨的质量逐渐增加。再生骨表现出具有骨细胞的成熟类骨基质结构。从机械性能方面来看，再生骨的质量与正常对照颅骨骨相似。结果表明，微压痕测试可用于评估初始再生和 Col 基质中 OCP 颗粒增强骨的再生骨质量。植入 OCP/Col 似乎可以作为确定再生骨质量的模型研究。
