Zhang Zhengwen W, Neu Björn
Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.
Biophys J. 2009 Aug 19;97(4):1031-7. doi: 10.1016/j.bpj.2009.06.006.
The adhesion of red blood cells (RBCs) to cells or surfaces is of current basic science and clinical interest yet the details governing this process are still being explored. In this study, the effects of nonadsorbing polymers on the adhesion of RBC to albumin-coated glass have been investigated employing interference reflection microscopy. Our experimental results indicate that adhesion can be induced in the presence of dextran with a molecular mass >or=70 kDa and that the induced forces are strong enough to significantly suppress membrane undulations. The overall dependence of the adhesion energies on the polymer concentration is consistent with the assumption that macromolecular depletion induces this attractive interaction. In conclusion, our results indicate that depletion interaction might play a significant role in RBC adhesion via initiating close contacts, and thus suggest the importance of depletion forces for RBC interactions and its relevance to a wide variety of in vitro and in vivo cell-cell and cell-surface interactions.
红细胞（RBC）与细胞或表面的黏附是当前基础科学和临床研究的热点，但该过程的具体细节仍在探索之中。在本研究中，我们采用干涉反射显微镜研究了非吸附性聚合物对红细胞黏附于白蛋白包被玻璃表面的影响。我们的实验结果表明，在分子量≥70 kDa的葡聚糖存在下可诱导黏附，且诱导力足以显著抑制膜波动。黏附能对聚合物浓度的总体依赖性与大分子排空诱导这种吸引力相互作用的假设一致。总之，我们的结果表明排空相互作用可能通过引发紧密接触在红细胞黏附中发挥重要作用，从而提示排空力对红细胞相互作用的重要性及其与多种体外和体内细胞-细胞及细胞-表面相互作用的相关性。
