Iqbal Munir, Xiao Hiaxia, Baillie Greg, Warry Andrew, Essen Steve C, Londt Brandon, Brookes Sharon M, Brown Ian H, McCauley John W
Institute for Animal Health, Compton Laboratory, Compton, Newbury, Berkshire RG20 7NN, UK.
Philos Trans R Soc Lond B Biol Sci. 2009 Sep 27;364(1530):2739-47. doi: 10.1098/rstb.2009.0088.
The emergence and spread of H5N1 avian influenza viruses from Asia through to Europe and Africa pose a significant animal disease problem and have raised concerns that the virus may pose a pandemic threat to humans. The epizootological factors that have influenced the wide distribution of the virus are complex, and the variety of viruses currently circulating reflects these factors. Sequence analysis of the virus genes sheds light on the H5N1 virus evolution during its emergence and spread, but the degree of virus variation at the level of an individual infected bird has been described in only a few studies. Here, we describe some results of a study in which turkeys, ducks and chickens were infected with either one of two H5N1 or one of three H7N1 viruses, and the degree of sequence variation within an individual infected avian host was examined. We developed 'deep amplicon' sequence analysis for this work, and the methods and results provide a background framework for application to disease outbreaks in the field.
H5N1禽流感病毒从亚洲出现并传播至欧洲和非洲，这构成了一个重大的动物疾病问题，并引发了人们对该病毒可能对人类构成大流行威胁的担忧。影响该病毒广泛传播的流行病学因素很复杂，目前流行的多种病毒反映了这些因素。对病毒基因进行序列分析有助于了解H5N1病毒在出现和传播过程中的进化情况，但仅有少数研究描述了单个感染禽类体内病毒的变异程度。在此，我们描述了一项研究的一些结果，该研究中用两种H5N1病毒之一或三种H7N1病毒之一感染火鸡、鸭和鸡，并检测了单个感染禽类宿主内的序列变异程度。我们为此项工作开发了“深度扩增子”序列分析方法，这些方法和结果为应用于现场疾病暴发提供了一个背景框架。
