Goddeeris B M, Morrison W I, Toye P G, Bishop R
International Laboratory for Research on Animal Diseases, Nairobi, Kenya.
Immunology. 1990 Jan;69(1):38-44.
To determine whether the major histocompatibility complex (MHC) phenotype of cattle could affect the parasite strain specificity of immunity to Theileria parva by influencing the antigenic specificity of Theileria-specific cytotoxic T lymphocytes (CTL), we investigated the parasite strain specificity of Theileria-specific CTL clones derived from cattle of different class I MHC phenotypes. Thirty-one class I-restricted CTL clones were generated from four cattle immunized with the Muguga stock of T. parva. The MHC restriction and parasite strain specificities were determined for each clone utilizing as targets, parasitized cell lines of different MHC phenotypes and cloned cell lines containing different parasite strains. CTL clones restricted by the same MHC determinant had similar parasite strain specificities. On the other hand, clones restricted by different MHC determinants exhibited different parasite strain specificities. This was true whether the clones were generated from the same animal or from different cattle and tested on a target cell line expressing both MHC determinants. These results provide strong evidence that differences in the strain specificities of CTL derived from animals immunized with the same parasite stock, are determined by the class I MHC phenotype of the immunized animal.
为了确定牛的主要组织相容性复合体（MHC）表型是否会通过影响泰勒虫特异性细胞毒性T淋巴细胞（CTL）的抗原特异性来影响对小泰勒虫免疫的寄生虫株特异性，我们研究了来自不同I类MHC表型牛的泰勒虫特异性CTL克隆的寄生虫株特异性。从用小泰勒虫穆古加株免疫的四头牛中产生了31个I类限制性CTL克隆。利用不同MHC表型的寄生虫细胞系和含有不同寄生虫株的克隆细胞系作为靶标，确定每个克隆的MHC限制性和寄生虫株特异性。受相同MHC决定簇限制的CTL克隆具有相似的寄生虫株特异性。另一方面，受不同MHC决定簇限制的克隆表现出不同的寄生虫株特异性。无论这些克隆是来自同一动物还是不同的牛，并在表达两种MHC决定簇的靶细胞系上进行测试，都是如此。这些结果提供了强有力的证据，表明用相同寄生虫株免疫的动物所产生的CTL的株特异性差异，是由免疫动物的I类MHC表型决定的。
