Won Kyu Yeoun, Park Hye-Rim, Park Yong-Koo
Department of Pathology, College of Medicine, Kyung Hee University, Seoul, Korea.
Tumori. 2009 May-Jun;95(3):311-6. doi: 10.1177/030089160909500307.
Osteosarcoma is the most common primary bone malignancy. Many genetic markers have proven prognostic value in osteosarcoma and studies are under way to determine their potential application as specific therapeutic targets. Runx2, Indian hedgehog (IHH), and Sox9 are proteins that play major roles in bone formation and tumorigenesis. We studied the protein expression of Runx2, IHH, and Sox9 in osteosarcoma and correlated their expression with clinicopathological variables. We also studied the prognostic value of the expression of these three genes in osteosarcoma.
We produced 48 formalin-fixed, paraffin-embedded tissue microarrays containing osteosarcoma tissue cores for immunohistochemical staining of Runx2, IHH and Sox9. We evaluated the expression of each gene by immunohistochemical staining and analyzed the relationship between expression and clinicopathological parameters.
High expression of Runx2 was significantly related to metastasis (P = 0.015). High expression of Runx2 indicated a trend toward a poor survival rate (P = 0.056). High expression of IHH and Sox9 were not related to any clinicopathological parameters.
High expression of Runx2 was significantly related to tumor metastasis in osteosarcoma. Our results suggest that overexpression of Runx2 might be a useful prognostic marker in osteosarcoma cases.
骨肉瘤是最常见的原发性骨恶性肿瘤。许多基因标志物已被证明在骨肉瘤中具有预后价值，并且正在进行相关研究以确定它们作为特定治疗靶点的潜在应用。Runx2、印度刺猬蛋白（IHH）和Sox9是在骨形成和肿瘤发生中起主要作用的蛋白质。我们研究了Runx2、IHH和Sox9在骨肉瘤中的蛋白表达，并将它们的表达与临床病理变量相关联。我们还研究了这三个基因的表达在骨肉瘤中的预后价值。
我们制作了48个包含骨肉瘤组织芯的福尔马林固定、石蜡包埋组织微阵列，用于Runx2、IHH和Sox9的免疫组织化学染色。我们通过免疫组织化学染色评估每个基因的表达，并分析表达与临床病理参数之间的关系。
Runx2的高表达与转移显著相关（P = 0.015）。Runx2的高表达表明生存率有降低的趋势（P = 0.056）。IHH和Sox9的高表达与任何临床病理参数均无关联。
Runx2的高表达与骨肉瘤中的肿瘤转移显著相关。我们的结果表明，Runx2的过表达可能是骨肉瘤病例中一个有用的预后标志物。
