Cao Jun, Jiang Liping, Geng Chengyan, Yao Xiaofeng
Laboratory of Toxicology, Dalian Medical University, Dalian 116044, China.
Wei Sheng Yan Jiu. 2009 Jul;38(4):392-5.
To investigate the protective effects of curcumin on acrylamide (AA)-induced DNA damage in HepG2 cells.
After pretreatment with curcumin (0.63, 1.25 and 2.50 microg/ml) for 1 hour, HepG2 cells were exposed to various concentrations of AA (0, 10, 20 mmol/L) for 1 h, comet assay was performed to determine DNA damage. The production of reactive oxygen species (ROS) was measured using the 2, 7-dichlorofluorescein diacetate (DCFH-DA) method.
One-hour exposure of HepG2 cells to AA led to a dose-dependent increase in DNA fragmentation. When the cells were pretreated with curcumin for 1 h, the comet tail moment values were decreased compared to only AA-treated cells, and curcumin of 2.50 microg/nml significantly decreased the comet tail moment values compared to only AA-treated cells (P < 0.05). The DCF fluorescence intensity increased when the HepG2 cells were treated with 10 and 20 mmol/L AA (P < 0.01). When the cells were pretreated with curcumin, the level of ROS was significantly decreased compared to only AA-treated (P < 0.01).
These data suggest that curcumin could attenuate AA-induced DNA damage in HepG2 cells. The protection is probably mediated by antioxidant protective mechanism.
研究姜黄素对丙烯酰胺（AA）诱导的HepG2细胞DNA损伤的保护作用。
用姜黄素（0.63、1.25和2.50微克/毫升）预处理1小时后，将HepG2细胞暴露于不同浓度的AA（0、10、20毫摩尔/升）中1小时，进行彗星试验以确定DNA损伤。采用2,7-二氯荧光素二乙酸酯（DCFH-DA）法测定活性氧（ROS）的产生。
HepG2细胞暴露于AA 1小时导致DNA片段化呈剂量依赖性增加。当细胞用姜黄素预处理1小时时，与仅用AA处理的细胞相比，彗星尾矩值降低，并且2.50微克/毫升的姜黄素与仅用AA处理的细胞相比显著降低了彗星尾矩值（P<0.05）。当HepG2细胞用10和20毫摩尔/升的AA处理时，DCF荧光强度增加（P<0.01）。当细胞用姜黄素预处理时，与仅用AA处理相比，ROS水平显著降低（P<0.01）。
这些数据表明姜黄素可以减轻AA诱导的HepG2细胞DNA损伤。这种保护作用可能是由抗氧化保护机制介导的。
