The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, People's Republic of China.
Biosci Rep. 2010 Apr 9;30(5):307-17. doi: 10.1042/BSR20090059.
A dimeric 64 kDa HA (haemagglutinin) was isolated with a high yield from dried Phaseolus vulgaris cultivar 'French bean number 35' seeds. It inhibited the proliferation of hepatoma HepG2 cells and breast cancer MCF-7 cells with an IC50 of 100 and 2 microM respectively. After exposure of MCF-7 cells to the HA for 24 h, a number of changes were detected in the cells. Growth arrest in the G0/G1 and G2/M phases was observed. The number of cells undergoing early apoptosis and late apoptosis increased. Disruption of the mitochondrial transmembrane potential and disorganization of the inner mitochondrial membrane were induced. Western-blot analysis disclosed that the HA induced apoptosis through the death receptor-mediated pathway.
从干燥的菜豆品种“法国 35 号”种子中高产量地分离出一种二聚体 64 kDa 的 HA（血凝素）。它抑制肝癌 HepG2 细胞和乳腺癌 MCF-7 细胞的增殖，IC50 分别为 100 和 2 μM。暴露于 HA 24 小时后，检测到 MCF-7 细胞发生了多种变化。观察到 G0/G1 和 G2/M 期的生长停滞。早期和晚期凋亡的细胞数量增加。诱导了线粒体跨膜电位的破坏和内部线粒体膜的紊乱。Western blot 分析表明，HA 通过死亡受体介导的途径诱导细胞凋亡。
